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hypervirulent laboratory strains  (ATCC)
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ATCC hypervirulent laboratory strains
Inducing the expression of episomal Wzc variants with L-arabinose increases sedimentation resistance in <t>hypervirulent</t> and classical Klebsiella strains. Wild-type Wzc and six mutants were expressed with the pBAD18 arabinose-inducible promoter in wild-type KPPR1 ( A and B ), K. variicola ( Kv ) 616 ( C and D ), K. pneumoniae ( Kp ) 714 ( E and F ), or K. quasipneumoniae ( Kqp ) 1346 ( G and H ). Empty vector (EV) was used as a negative control. The strains were cultured in LB medium containing 50 mM L-arabinose and kanamycin. ( A, C, E and G ) Mucoidy was determined by quantifying the supernatant OD 600 after sedimenting 1 OD 600 unit of culture at 1,000 × g for 5 min. ( B, D, F and H ) Uronic acid content of the total culture or supernatant was quantified and normalized to the OD 600 of the overnight culture. The cell-associated CPS was deduced by subtracting the cell-free uronic acid content (EPS) from the total culture uronic acid content. Data presented are the mean, and error bars represent the standard error of the mean. Statistical significance was determined using two-way ANOVA with a Bonferroni post-test to compare specific groups. * P < 0.0332; ** P < 0.021; *** P < 0.0002; # P < 0.0001. Experiments were performed ≥3 independent times, in triplicate.
Hypervirulent Laboratory Strains, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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st439 k2 hypervirulent k pneumoniae atcc 43816  (ATCC)
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ATCC st439 k2 hypervirulent k pneumoniae atcc 43816
Inducing the expression of episomal Wzc variants with L-arabinose increases sedimentation resistance in <t>hypervirulent</t> and classical Klebsiella strains. Wild-type Wzc and six mutants were expressed with the pBAD18 arabinose-inducible promoter in wild-type KPPR1 ( A and B ), K. variicola ( Kv ) 616 ( C and D ), K. pneumoniae ( Kp ) 714 ( E and F ), or K. quasipneumoniae ( Kqp ) 1346 ( G and H ). Empty vector (EV) was used as a negative control. The strains were cultured in LB medium containing 50 mM L-arabinose and kanamycin. ( A, C, E and G ) Mucoidy was determined by quantifying the supernatant OD 600 after sedimenting 1 OD 600 unit of culture at 1,000 × g for 5 min. ( B, D, F and H ) Uronic acid content of the total culture or supernatant was quantified and normalized to the OD 600 of the overnight culture. The cell-associated CPS was deduced by subtracting the cell-free uronic acid content (EPS) from the total culture uronic acid content. Data presented are the mean, and error bars represent the standard error of the mean. Statistical significance was determined using two-way ANOVA with a Bonferroni post-test to compare specific groups. * P < 0.0332; ** P < 0.021; *** P < 0.0002; # P < 0.0001. Experiments were performed ≥3 independent times, in triplicate.
St439 K2 Hypervirulent K Pneumoniae Atcc 43816, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 99 stars, based on 1 article reviews
st439 k2 hypervirulent k pneumoniae atcc 43816 - by Bioz Stars, 2026-10
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Inducing the expression of episomal Wzc variants with L-arabinose increases sedimentation resistance in hypervirulent and classical Klebsiella strains. Wild-type Wzc and six mutants were expressed with the pBAD18 arabinose-inducible promoter in wild-type KPPR1 ( A and B ), K. variicola ( Kv ) 616 ( C and D ), K. pneumoniae ( Kp ) 714 ( E and F ), or K. quasipneumoniae ( Kqp ) 1346 ( G and H ). Empty vector (EV) was used as a negative control. The strains were cultured in LB medium containing 50 mM L-arabinose and kanamycin. ( A, C, E and G ) Mucoidy was determined by quantifying the supernatant OD 600 after sedimenting 1 OD 600 unit of culture at 1,000 × g for 5 min. ( B, D, F and H ) Uronic acid content of the total culture or supernatant was quantified and normalized to the OD 600 of the overnight culture. The cell-associated CPS was deduced by subtracting the cell-free uronic acid content (EPS) from the total culture uronic acid content. Data presented are the mean, and error bars represent the standard error of the mean. Statistical significance was determined using two-way ANOVA with a Bonferroni post-test to compare specific groups. * P < 0.0332; ** P < 0.021; *** P < 0.0002; # P < 0.0001. Experiments were performed ≥3 independent times, in triplicate.

Journal: mSphere

Article Title: Urine-mediated suppression of Klebsiella pneumoniae mucoidy is counteracted by spontaneous Wzc variants altering capsule chain length

doi: 10.1128/msphere.00288-23

Figure Lengend Snippet: Inducing the expression of episomal Wzc variants with L-arabinose increases sedimentation resistance in hypervirulent and classical Klebsiella strains. Wild-type Wzc and six mutants were expressed with the pBAD18 arabinose-inducible promoter in wild-type KPPR1 ( A and B ), K. variicola ( Kv ) 616 ( C and D ), K. pneumoniae ( Kp ) 714 ( E and F ), or K. quasipneumoniae ( Kqp ) 1346 ( G and H ). Empty vector (EV) was used as a negative control. The strains were cultured in LB medium containing 50 mM L-arabinose and kanamycin. ( A, C, E and G ) Mucoidy was determined by quantifying the supernatant OD 600 after sedimenting 1 OD 600 unit of culture at 1,000 × g for 5 min. ( B, D, F and H ) Uronic acid content of the total culture or supernatant was quantified and normalized to the OD 600 of the overnight culture. The cell-associated CPS was deduced by subtracting the cell-free uronic acid content (EPS) from the total culture uronic acid content. Data presented are the mean, and error bars represent the standard error of the mean. Statistical significance was determined using two-way ANOVA with a Bonferroni post-test to compare specific groups. * P < 0.0332; ** P < 0.021; *** P < 0.0002; # P < 0.0001. Experiments were performed ≥3 independent times, in triplicate.

Article Snippet: To examine the effect of the urinary tract environment on K. pneumoniae mucoidy and CPS production, five strains were selected: two hypervirulent laboratory strains, KPPR1 (rifampin-resistant ATCC 43816 derivative) and NTUH-K2044, and three classical strains recently isolated from clinical UTI cases, 616, 714, and 1346 ( ).

Techniques: Expressing, Sedimentation, Plasmid Preparation, Negative Control, Cell Culture